rabbit anti human pjak2  (Cell Signaling Technology Inc)

Journal: Scientific Reports

Article Title: Pacritinib inhibits proliferation of primary effusion lymphoma cells and production of viral interleukin-6 induced cytokines

doi: 10.1038/s41598-024-54453-7

Figure Lengend Snippet: Pacritinib inhibits activities of JAK2, STAT3 and NF-κB. Protein extracts of JSC-1 and BCBL-1 cells treated for 48 h with 1 μM pacritinib (CTI BioPharma Corp.) or DMSO control were analyzed for the total and phosphorylated JAK2, STAT3 and p65 of NF-κB by Western blot. ( A ) JAK2, STAT3 and p65 indicate the total amount of the protein; pJAK2, pSTAT3 and p65 indicate the phosphorylated forms of the proteins, and β-actin serves as the internal control. Intensity of each band in JSC-1 group. Western blots were stripped after the initial probe of the phosphorylated proteins and β-actin and then probed with antibodies to the unphosphorylated proteins. The full scanned Western blots of Fig. 3A are provided in Supplemental File 12. ( B ) Signal intensities for JSC-1 and ( C ) BCBL-1 cells were normalized according to β-actin, and signal of all total and phosphorylated JAK2, STAT3 and p65 of NF-κB in the Pac-treated cells were presented as percentages compared with that of the control cells.

Article Snippet: Primary antibodies used were: mouse anti-β-actin (Sigma, cat# A2228), rabbit anti-human JAK2 (Cell Signaling, cat# 3230S), rabbit anti-human pJAK2 (Cell Signaling, cat# 3771S), rabbit anti-human STAT3 (Cell Signaling, cat# 12640S), rabbit anti-human pSTAT3 (Cell Signaling, cat# 9145S), rabbit anti-human p65 (Cell Signaling, cat# 8242S), mouse anti-human pp65 (Cell Signaling, cat# 3043S), and rabbit anti-human FLT3 (Cell Signaling, cat# 3462S), rabbit anti-human pFLT3 (Cell Signaling, cat# 3463S).

Techniques: Control, Western Blot